Phis2.1 vector

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August undefined, 2024

http://www.yrgene.com/documents/vector/matchmaker_library_constructionscreening_kits_user_manual.pdf Webb26 mars 2024 · The promoter sequence (1800 bp) of MdMATE8 (MDP0000175055) was inserted into the pHIS2.1 vector (Clontech, Palo Alto, CA, United States), which …

pHis2酵母表达载体说明.pdf

Webb12 apr. 2024 · 目的：筛选与启动子pHIS2-A互作的蛋白。. 方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。. 材料：. 1．启动子克 … Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, … iphone 5s best price

(PDF) Exploring transcriptional signalling mediated by OsWRKY13, …

http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 WebbpHis2载体价格1000元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。 Webb25 okt. 2016 · In the mutated assay, the sequence of the G-box in the NWRE (GCGTTCTTACTTTTCGTAAGGG CACGTG AAGCACCCCAACAAA) was replaced with … iphone 5s bluetooth 4.0

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WebbWe'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2024 ... Webb20 feb. 2024 · The full-length cDNA of TcbZIP60 was cloned into the pGADT7 vector used for homologous recombination, and the promoter sequences of TcCHS and TcAOC were cloned separately into the pHis2.1 vector. The resultant pGADT7: TcbZIP60 plasmid was co-transformed into yeast strain Y187, together with pHis2.1: TcCHS or pHis2.1: TcAOC , … iphone 5s bluetooth 4Webb21 mars 2024 · 其中，香豆素（1,2-苯并吡喃酮）的生理浓度足以抑制苹果枝在hk组织中的生长，外源施用可以提高lk苹果枝条对苹果枝的抗性。过表达β-葡萄糖苷酶40（MdBGLU40）的转基因苹果愈伤组织编码香豆素合成酶，含有更高水平的香豆素，即使在LK条件下也表现出对苹果枯萎病的高抗性。 iphone 5s bluetooth 3

"Webb16 okt. 2014 · Specifically, the bait construct pHis2.1−2 × 150 was generated by cloning tandem copies of the 150-bp enhancer (2 × 150) into the MluI site of pHis2.1, a one … " - Phis2.1 vector

Phis2.1 vector

3-AT(3-氨基-1,2,4-三唑)（ZCS104）-北京庄盟国际生物基因科技有 …

http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html WebbpGreenII 0800-LUC (VET1290) - Creative Biogene Home Products Clones Vectors Expression Vector pGreenII 0800-LUC pGreenII 0800-LUC Cat.No. VET1290 Host Cell plant, Escherichia coli Promoter CaMV 35S Resistance Kanamycin Vector Size 6382 bp Vector Type Expression Vector Q&A Customer Reviews Related Products For research …

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WebbUnique restriction sites are in bold. pHIS2.1 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was … http://www.biofeng.com/zaiti/jiaomu/pYES2.html

Webb26 maj 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for … Webb11 juli 2014 · 常用载体及通用测序引物,测序通用引物,华大测序通用引物,载体通用引物,petduet 1测序引物,通用引物,测序引物,pet28a测序引物,m13通用引物,细菌通用引物,病毒通用引物. VectorPrimer (F)Primer (R)抗性载体大小 (bp)EZ-TM13-47RV-MAmp+2984LZRS-RfALZRS-FLZRS-R11100p3xFLAGMV14CMV30 (834bp)CMV24 ...

Webb16 feb. 2024 · Two GA-inducible genes expansin 1 (PhEXP1) and cysteine proteinase 1 (PhCP1) were down-regulated in the flowers infected with TRV-PhCHS/GA20ox3 . Expression levels of PhSAG12 and PhSAG29 in TRV- PhCHS / GA20ox3 -infected corollas were detected to be significantly higher than those in the corollas infected with the … WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. …

WebbVectors in Use. N or C-terminal His tag. Parallel vectors 1 & 3 contain N-terminal His (pET22), MBP (pMal) and GST (pGex) with similar MCS for easy cloning. Parallel vector 2 contains N and C-terminal His (pET22), …

Webb9 apr. 2024 · The recombinant vectors were transferred into yeast strain Y187 cells using the LiAC-PEG4000 method. The TF-encoding genes were amplified and inserted into the pGADT7 vector, and the resulting constructs were then transformed into the pHis2 yeast strain containing the promoter plasmid. iphone 5s bluetooth pairingWebb使用方法: 1.计算所需平板的数量，计算配置培养基体积；. 2.设置3-AT的浓度梯度（建议10-50 mM 的浓度范围设3-5个梯度，常用浓度约30 mM）；. 3.灭菌筛选培养基，待培养基冷 … iphone 5s board replacementWebb5 juli 2024 · The empty vector pHIS2.1 was used as the negative control. All cells carrying different vector combinations grew normally and equally on the SD/-Leu/-Trp medium (Fig. 4 b). However, the growth of yeast cells with the combination of pGADT7/pHIS2.1 empty vectors or pGADT7- GsERF71 /pHIS2.1 vectors was significantly inhibited on the SD/ … iphone 5s bluetooth upgradeWebbpYES2的是一个5.9 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。. 载体的特点在于基因插入载体的构建简单，以及能够使用原养型尿嘧啶进 … iphone 5s boot loop after screen replacementWebbA yeast one-hybrid library was constructed to screen the binding proteins of suspensor specific cis-element. In this study, five repeats of suspensor specific cis-element … iphone 5s boots on muteWebb29 okt. 2024 · DNA-protein interactions were verified by growth performance of yeast cells co-transformed with the indicated pairs of Rec2 and pHis2.1 vectors on synthetic defined (SD) medium –Leu–Trp–His containing 3-amino-1,2,4-triazole (3-AT). iphone 5s bluetooth headsetWebb19 sep. 2024 · The pHIS2 vector carries the TRP1 nutritional marker for selection in yeast. The transformed yeast cells were cultured on SD/-Trp-Leu medium and SD/-Trp-His-Leu medium supplemented with 10 mM or 30 mM 3-amino … iphone 5s bluetooth smart