Cisplatin binding to holliday junction

Author: sprk

August undefined, 2024

WebFeb 1, 2003 · When the synthetic Holliday junction was present along with the half-cruciform and double-stranded oligonucleotides, the Rad51B protein only bound to the synthetic Holliday junction, which... WebMay 15, 2007 · This binding mode is specific for the Holliday junction and does not cause unwinding of the DNA helices. Citing Literature Supporting Information Volume 46, Issue …

Cisplatin (Cisplatin Injection): Uses, Dosage, Side Effects ... - RxList

WebJul 5, 2024 · Holliday junctions (HJs) are an important class of nucleic acid structure utilized in DNA break repair processes. As such, these structures have great importance as therapeutic targets and for... WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … high school ai

Holliday junction cleavage by poxvirus resolvase and DNA …

WebJul 11, 2000 · Actually, both of the RuvA tetramers, the upper and the lower, simultaneously contact each strand of a DNA duplex arm ( 16 ). By contrast, in complex I, the two … WebTo expose two cut sites, the Holliday junction is rotated by 180° to form a sequare planar structure. Resolution of Holliday junction occurs by cutting the DNA strands at the site of cross and re-joining them. Resolution occurs in one of the two ways. This gives rise to two classes of DNA products. WebHolliday junctions (Holliday, 1964) are four-way junctions in DNA formed by the mutual exchange of strand connectivity, and thus connected by the covalent continuity of each strand of the junction ( Orr-Weaver, Szostak, & Rothstein, 1981; Potter & Dressler, 1976; Schwacha & Kleckner, 1995 ). high school air force rotc scholarship

Ligand Bridging of the DNA Holliday Junction: Molecular …

Crystal structure of the Holliday junction DNA in complex with a …

WebJun 3, 2024 · Wang, W. et al. Holliday junction thermodynamics and structure: coarse-grained simulations and experiments. Sci. Rep. 6, 22863 (2016). WebSep 15, 2024 · Cisplatin (Cisplatin Injection) may treat, side effects, dosage, drug interactions, warnings, patient labeling, reviews, and related medications including drug … how many carbs in monk fruit sugarWebThe binding of Holliday junction DNA by endo-exonuclease. Purified endo-exonuclease (5 units) was incubated with 32P-labeled junction molecules, linear ss-DNA (146 nucleotides), and linear ds-DNA ... high school aid

"WebFeb 21, 1997 · The E. coli RuvA and RuvB proteins, which are involved in the late stages of recombination and the recombinational repair of damaged DNA, bind to Holliday junctions and promote branch migration. We have used electron microscopy and image analysis to examine RuvA and RuvB bound to model Holliday structures. " - Cisplatin binding to holliday junction

Cisplatin binding to holliday junction

Search and processing of Holliday junctions within long …

Webway DNA structure, also called a Holliday junction, which is processed by the RuvABC proteins.2 The RuvA protein initially binds the Holliday junction, and then recruits RuvB protein rings to opposite sides of the junction.3,4 RuvB is a molecular motor that uses the energy derived from ATP binding and hydrolysis to drive unidirectional movement ... WebThe RuvAB complex can carry out DNA helicase activity, which helps unwind the duplex DNA. The binding of the RuvC protein to the RuvAB complex is thought to cleave the …

Did you know?

WebAbstract. In the present paper, the interactions of the origin binding protein (OBP) of herpes simplex virus type 1 (HSV1) with synthetic four-way Holliday junctions (HJs) were … WebIt has been found that Thermus thermophilus RuvC (TthRuvC) binds specifically to an unstacked Holliday junction and cuts selective DNA sequence, while poxvirus HJ resolvase does not have strong...

WebJul 2, 2004 · The RuvC protein binds specifically to a Holliday junction and cleaves it in one of the two possible orientations [16], [17], [18]. No spontaneous or cisplatin-induced … WebJan 4, 2024 · Organometallic Pillarplexes that bind DNA 4-way Holliday Junctions and Forks bioRxiv. bioRxiv posts many COVID19-related papers. A reminder: they have not …

WebThe BCDX2 complex also bound to the synthetic Holliday junction, and its af®nity to the Holliday-junction DNA was signi®cantly higher than that of Rad51B (Fig. 4). WebAug 5, 2011 · Holliday junctions are formed during recombination, double-strand break repair, and fork reversal during replication. Resolving Holliday junctions is a critical process for maintaining genomic stability [ 25, 26 ]. These junctions are resolved by a class of structure-specific nucleases: the junction-resolving enzymes. Figure 1

WebConformational switching of the synthetic Holliday junction (HJ) labeled with a Cy3-Cy5 fluorophore pair. (b) Representative single molecule fluorescence-time traces from our …

WebNational Center for Biotechnology Information high school air force academyWebMar 15, 1997 · The results confirm that RuvC protein binds Holliday junctions as a dimer. Influence of DNA sequence on incision Previously it was shown that Holliday junction cleavage by RuvC occurs at specific sequences, with the consensus defined as 5′- A / T TT ↓G / C -3′. Single base changes within this sequence severely reduce the efficiency of … how many carbs in monk fruit sweetener how many carbs in moo shu porkWebAug 27, 2024 · Brogden, A.L.; Hopcroft, N.H.; Searcey, M.; Cardin, C.J. Ligand bridging of the DNA Holliday junction: Molecular recognition of a stacked-X four-way junction by a … high school air rifleWebK Komori, S Sakae, H Shinagawa, K Morikawa, Y Ishino, A Holliday junction resolvase from Pyrococcus furiosus: Functional similarity to Escherichia coli RuvC provides … how many carbs in monterey jack cheeseWebCX3 (A) or BCDX2 (B) was incubated with Holliday junction templates, mounted onto carbon-coated copper grids, and rotary shadowcast with tungsten for visualization by EM. Images are shown in ... how many carbs in mott\u0027s applesauceWebApr 3, 2007 · The bifunctional binding of the anticancer drug cisplatin to two adjacent nucleobases in DNA is modeled using density functional theory. Previous experimental studies revealed that cisplatin binding to adjacent guanine and adenine is sensitive to nucleobase sequence. high school aizawa